A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. Wang, Ying-Chih Singh, Anup K.Įmbodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. (Contains 8 figures and 4 tables.)ĭevices, systems, and methods for microscale isoelectric fractionation The main object of this work is to present the pedagogical usefulness of the theoretical methods, developed in this laboratory, for the determination of the isoelectric point (pI) and the net electric charge of proteins together with some comments on the naming of the acid-base residues of proteins. Isoelectric Point, Electric Charge, and Nomenclature of the Acid-Base Residues of ProteinsĮRIC Educational Resources Information Center We further consider various models of the origin of the charge on nonpolar material-water interfaces. IEPs of viruses are found in pH range from 1.9 to 8.4 most frequently, they are measured in a band of 3.5 C8) alkane oils, as well as nitrogen and hydrogen gases, all in various simple 1:1 electrolyte solutions confirm the general validity of the result. Here, we review the IEP measurements of viruses that replicate in hosts of kingdom plantae, bacteria and animalia. The pH value at which the net surface charge switches its sign is referred to as the isoelectric point (abbreviations: pI or IEP) and is a characteristic parameter of the virion in equilibrium with its environmental water chemistry. This electrostatic charge determines the mobility of the soft particle in an electric field and thus governs its colloidal behaviour which plays a major role in virus sorption processes. Viruses as well as other (bio-)colloids possess a pH-dependent surface charge in polar media such as water. The value of the isoelectric point corresponded well with cell surface hydrophobicity, as well as with the ability to form biofilm in these yeasts. The isoelectric points of 39 Candida parapsilosis strains were determined by means of capillary isoelectric focusing. Ruzicka, Filip Horka, Marie Hola, Veronika Kubesova, Anna Pavlik, Tomas Votava, Miroslav The differences in the isoelectric points of biofilm-positive and biofilm-negative Candida parapsilosis strains.
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